It is always god idea to segment your ISCSI traffic with LAN. Here are some of the best practices
1. Enable Port fast on the switches
2. Enable Spanning tree protocol
3. enable Flow control
4. Enable Jumbo frames if your SAN supports, jumbo frames should be enabled on SAN, switch as well NIC
5. Use Vlan for ISCSI traffic incase if you use the same switches for your LAN as well
It is always good idea to have 3 NIc's in the server where 1 NIC can be used for LAN and the other two NIC's can be used for redundancy and also load balancing depending on your SAN compatibility